Antisickling and Antioxidant Properties of Omega-3 Fatty Acids EPA/DHA

 


Abstract

Background: Therapeutic treatment of Sickle cell disease (SCD) is complex and very expensive. However, natural products have been used to manage sickle cell crises. Omega-3 fatty acids EPA/DHA reduce the number of crisis in SCD.

Objectives: This study aimed to assess the antisickling and antioxidant properties of omega-3 fatty acids EPA/DHA.

Methods: The evaluation of the rates of inhibition of induced sickling by sodium metabisulfite 2% and the potential reversal of sickle cells into normal spherical erythrocytes was performed using microscopic enumeration of red blood corpuscles of the sickling. The evaluation of membrane stability effect, FRAP, DPPH°, and OH° assays was determined using colorimetric method.

Results: Sodium metabisulfite increased the sickling of RBCs from 26.3±1.6 to 79.42±5.2% during 3 hours. Omega-3 fatty acids EPA/DHA 0.2% showed the best antisickling (79.05±1.2%) and reversibility (64.82± 2.7%) rate; the best membrane stability compared to other concentrations. Omega-3 fatty acids EPA/DHA revealed an appreciable reducing power at 26.08±0.3mg FeII/100g. It also showed an inhibitory activity on free radicals of DPPH and hydroxyl radical at IC50 3.7±0.0 and 11.28±0.3mg/mL respectively.

Conclusion: Omega-3 fatty acids EPA/DHA have antisickling, anti-haemolytic and antioxidant properties. The results obtained are in addition to those of the authors who showed that Omega-3 fatty acids EPA/DHA reduce the number of crisis in SCD.


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